Transposon Tn5 excision in yeast: influence of DNA polymerases alpha, delta, and epsilon and repair genes.
نویسندگان
چکیده
منابع مشابه
Adjacent sequences influence DNA repair accompanying transposon excision in maize.
Mobile elements transposing via DNA intermediates often leave small rearrangements, or "transposon footprints," at sites where they excise. Each excision event leaves its own footprint and, at any given site, these vary in size and sequence. Footprint formation involves DNA repair of sequences flanking the element. We have analyzed the footprints formed by a 2-kb Ds element excising from six di...
متن کاملAccessibility of DNA polymerases to repair synthesis during nucleotide excision repair in yeast cell-free extracts.
Nucleotide excision repair (NER) removes a variety of DNA lesions. Using a yeast cell-free repair system, we have analyzed the repair synthesis step of NER. NER was proficient in yeast mutant cell-free extracts lacking DNA polymerases (Pol) beta, zeta or eta. Base excision repair was also proficient without Polbeta. Repair synthesis of NER was not affected by thermal inactivation of the tempera...
متن کاملRole of DNA polymerases in excision repair in Escherichia coli.
The excision repair process following ultraviolet irradiation has been fractionated into its individual steps in toluene-treated cells. Incision can be examined in vitro independently of other reactions by omission of the deoxyribonucleoside triphosphates which prohibits repair synthesis and causes incisions to accumulate. Incision requires ATP, continues from 10 to 15 min at 37”, and is specif...
متن کاملLong patch base excision repair with purified human proteins. DNA ligase I as patch size mediator for DNA polymerases delta and epsilon.
Among the different base excision repair pathways known, the long patch base excision repair of apurinic/apyrimidinic sites is an important mechanism that requires proliferating cell nuclear antigen. We have reconstituted this pathway using purified human proteins. Our data indicated that efficient repair is dependent on six components including AP endonuclease, replication factor C, proliferat...
متن کاملWhich DNA polymerases are used for DNA-repair in eukaryotes?
There are five well-characterized nuclear DNA polymerases in eukaryotes (DNA polymerases alpha, beta, delta, epsilon and zeta) and this short review summarizes our current knowledge concerning the participation of each in DNA-repair. The three major DNA excision-repair pathways involve a DNA synthesis step that replaces altered bases or nucleotides removed during repair. Base excision-repair re...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1992
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.89.9.3785